BOISSEAUX Paul
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Scientist Theme(s) : Phone: +33 2 23 48 52 37 Email : paul.boisseaux@inrae.fr Address: UMR DECOD, 65 rue de Saint-Brieuc, Bât. 15, CS 84215, 35042 Rennes Cedex, France Keywords: Ecotoxicology, Immunotoxicology, Proteomics, Biomarkers, Cellular biology, Eco-evolution, Freshwater gastropod, Bioinformatics |
Expertise
I have Research experience in environmental toxicology, ecotoxicology and immunotoxicology : from molecular to cellular and organismal levels (life-history traits).
My PhD thesis was based in the domain of ecotoxicology, with a focus on environmental immunotoxicological aspects of a wide variety of contaminants in several populations of the freshwater gastropod Lymnaea stagnalis).
Current Position
Postdoctoral researcher, EPIX (Evolutionary ecology of systems Perturbed by biological Invasions and Xenobiotics) team, ESE research unit
Research
Current Research Topic : «Exploration of ecotoxico-proteomic biomarkers in the freshwater gastropod Lymnaea stagnalis, with eco-evolutionary insights, in response to Diquat herbicide exposure».
Background
L. stagnalis is a freshwater gastropod species. It is an ecologically relevant model representative of many lentic systems in holarctic regions. It has been studied in ecotoxicology for decades and an OECD reprotoxicity test has been validated (test n°243). Nowadays, high-throuput shotgun proteomics by means of next-generation sequencing mass spectrometers allow very promising applications in ecotoxicology. For instance, it provides a better comprehension of xenobiotics modes of action (e.g., adverse outcome pathways, AOPs) and it can be used more pragmatically for biomonitoring of aquatic environments (multiplexed health and/or exposure biomarkers).
Objectives
The first objective of the project is to characterize the proteome of L. stagnalis’ digestive gland and hemolymphatic tissues (plasma and hemocytes which are the circulating immunocompetent cells) in adult specimens. This work will contribute to ameliorate proteogenomic annotations in the L. stagnalis genome, in support of the STAGING project, a multidisciplinary scientific consortium
The second objective is to select candidate proteomic biomarkers for use in ecotoxicology, notably for identifying AOPs within the OECD framework. To that aim, I am currently studying molecular responses in L. stagnalis at the proteomic level following short-term exposure to Diquat (authorized herbicide in EU with a wide action spectrum), at environmentally worst-case but realistic concentrations.
Finally, the third objective is to gain evolutionary insights into the molecular aspects governing L. stagnalis’ responses to exotoxicological stressor. Several genetically-selected strains of laboratory-bred organisms will be used : strains pre-exposed to diquat in previous generations, as well as strains reproducing either by self- or cross-fertilization (L. stagnalis is a simultaneous hermaphroditic species). Related framing questions are : Do transgenerational effects of diquat pre-exposure occur at the proteomic level following short-term exposure to diquat in subsequent generations ? Is inbreeding depression influencing proteomic responses to short-term toxic insult in L. stagnalis?
Collaborations
CEA-Marcoule, DRF-Li2D: Jean ARMANGAUD and Christine ALMUNIA
Laboratory “Innovative technologies for Detection and Diagnostics”